Operating Room Cleaning Checklist

An operating room cleaning checklist is a detailed list of tasks to ensure that the surgical environment is sterile and safe for patients.

Pre-Operation

  • Inspect and organize clean supplies
  • Check functionality of equipment
  • Disinfect high-touch surfaces
  • Prepare sterile instruments and trays
  • Stock hand sanitizers and wipes
  • Set up waste disposal bins
  • Ensure proper ventilation
  • Verify surgical light cleanliness

Between Cases

  • Remove used instruments
  • Clean and disinfect surfaces
  • Dispose of waste appropriately
  • Replace linens and drapes
  • Mop and disinfect floor
  • Restock necessary supplies
  • Sanitize patient positioning tools
  • Inspect and clean anesthesia equipment

End of Day

  • Thoroughly disinfect all surfaces
  • Deep clean floors
  • Sanitize walls and ceilings
  • Clean and sterilize instruments
  • Empty and disinfect waste bins
  • Wipe down and disinfect light handles
  • Store all equipment properly
  • Check and restock supplies

Weekly Deep Clean

  • Deep clean all surfaces and fixtures
  • Inspect and sanitize air vents
  • Clean and polish stainless steel
  • Replace air filters
  • Sanitize storage cabinets
  • Disinfect computer and electronics
  • Check for wear and tear on equipment
  • Replenish emergency supplies